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CH Instruments chi-square test
Chi Square Test, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriginLab corp originpro 2022b
Experimental roadmap for the fabrication of the anatomical gingival tissue model. The workflow comprised of four steps. A) Silk proteins: silk solutions were obtained from B. mori silkworm cocoons, cut into small pieces, and boiled for 30 min; the fibers were dissolved, and the resulting solution was dialyzed against water and centrifuged to remove impurities. The final solution was then stored at 4 °C before use. B) Type I Collagen: type I Collagen isolated from rat tail was prepared by mixing DMEM and collagen solution and then neutralized by NaOH before use. C) Anatomical gingival tissue model: a SolidWorks rendering of a human gingival structure was generated to recreate a subsequent PDMS mold into which to cast the silk gingival scaffold (scale bar = 1 mm); the silk solution was pipetted into the PDMS mold and freeze‐dry to obtain a porous structure; subsequently, the scaffold was autoclaved to induce changes in the protein secondary structures, and 3D printed dental resin teeth were inserted into the scaffold to create the <t>periodontal</t> pocket. D) Overall approach: the complete construct was characterized by combining the three elements described above to mimic the in vivo features of gingival tissue. Briefly, human gingival fibroblasts were embedded in collagen I and seeded into the scaffold to replicate connective tissue; next, human gingival epithelial cells were seeded on the apical portion of the scaffold to replicate epithelial tissue. Finally, dental resin teeth were implanted to recreate the periodontal pocket in which the healthy microbiome, isolated from subgingival plaque, was inoculated to initiate host–pathogen interactions in vitro. Created with BioRender.com.
Originpro 2022b, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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originpro 2022b - by Bioz Stars, 2026-10
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STATA Corporation χ 2 test
Experimental roadmap for the fabrication of the anatomical gingival tissue model. The workflow comprised of four steps. A) Silk proteins: silk solutions were obtained from B. mori silkworm cocoons, cut into small pieces, and boiled for 30 min; the fibers were dissolved, and the resulting solution was dialyzed against water and centrifuged to remove impurities. The final solution was then stored at 4 °C before use. B) Type I Collagen: type I Collagen isolated from rat tail was prepared by mixing DMEM and collagen solution and then neutralized by NaOH before use. C) Anatomical gingival tissue model: a SolidWorks rendering of a human gingival structure was generated to recreate a subsequent PDMS mold into which to cast the silk gingival scaffold (scale bar = 1 mm); the silk solution was pipetted into the PDMS mold and freeze‐dry to obtain a porous structure; subsequently, the scaffold was autoclaved to induce changes in the protein secondary structures, and 3D printed dental resin teeth were inserted into the scaffold to create the <t>periodontal</t> pocket. D) Overall approach: the complete construct was characterized by combining the three elements described above to mimic the in vivo features of gingival tissue. Briefly, human gingival fibroblasts were embedded in collagen I and seeded into the scaffold to replicate connective tissue; next, human gingival epithelial cells were seeded on the apical portion of the scaffold to replicate epithelial tissue. Finally, dental resin teeth were implanted to recreate the periodontal pocket in which the healthy microbiome, isolated from subgingival plaque, was inoculated to initiate host–pathogen interactions in vitro. Created with BioRender.com.
χ 2 Test, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-sample+t-test+sas+procedure+proc+glm/STATA+2%2E0/pmc04037234-97-11-53
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Experimental roadmap for the fabrication of the anatomical gingival tissue model. The workflow comprised of four steps. A) Silk proteins: silk solutions were obtained from B. mori silkworm cocoons, cut into small pieces, and boiled for 30 min; the fibers were dissolved, and the resulting solution was dialyzed against water and centrifuged to remove impurities. The final solution was then stored at 4 °C before use. B) Type I Collagen: type I Collagen isolated from rat tail was prepared by mixing DMEM and collagen solution and then neutralized by NaOH before use. C) Anatomical gingival tissue model: a SolidWorks rendering of a human gingival structure was generated to recreate a subsequent PDMS mold into which to cast the silk gingival scaffold (scale bar = 1 mm); the silk solution was pipetted into the PDMS mold and freeze‐dry to obtain a porous structure; subsequently, the scaffold was autoclaved to induce changes in the protein secondary structures, and 3D printed dental resin teeth were inserted into the scaffold to create the periodontal pocket. D) Overall approach: the complete construct was characterized by combining the three elements described above to mimic the in vivo features of gingival tissue. Briefly, human gingival fibroblasts were embedded in collagen I and seeded into the scaffold to replicate connective tissue; next, human gingival epithelial cells were seeded on the apical portion of the scaffold to replicate epithelial tissue. Finally, dental resin teeth were implanted to recreate the periodontal pocket in which the healthy microbiome, isolated from subgingival plaque, was inoculated to initiate host–pathogen interactions in vitro. Created with BioRender.com.

Journal: Advanced Science

Article Title: Three‐Dimensional Humanized Model of the Periodontal Gingival Pocket to Study Oral Microbiome

doi: 10.1002/advs.202205473

Figure Lengend Snippet: Experimental roadmap for the fabrication of the anatomical gingival tissue model. The workflow comprised of four steps. A) Silk proteins: silk solutions were obtained from B. mori silkworm cocoons, cut into small pieces, and boiled for 30 min; the fibers were dissolved, and the resulting solution was dialyzed against water and centrifuged to remove impurities. The final solution was then stored at 4 °C before use. B) Type I Collagen: type I Collagen isolated from rat tail was prepared by mixing DMEM and collagen solution and then neutralized by NaOH before use. C) Anatomical gingival tissue model: a SolidWorks rendering of a human gingival structure was generated to recreate a subsequent PDMS mold into which to cast the silk gingival scaffold (scale bar = 1 mm); the silk solution was pipetted into the PDMS mold and freeze‐dry to obtain a porous structure; subsequently, the scaffold was autoclaved to induce changes in the protein secondary structures, and 3D printed dental resin teeth were inserted into the scaffold to create the periodontal pocket. D) Overall approach: the complete construct was characterized by combining the three elements described above to mimic the in vivo features of gingival tissue. Briefly, human gingival fibroblasts were embedded in collagen I and seeded into the scaffold to replicate connective tissue; next, human gingival epithelial cells were seeded on the apical portion of the scaffold to replicate epithelial tissue. Finally, dental resin teeth were implanted to recreate the periodontal pocket in which the healthy microbiome, isolated from subgingival plaque, was inoculated to initiate host–pathogen interactions in vitro. Created with BioRender.com.

Article Snippet: Two‐ sample Student’ t ‐test, one‐way ANOVA and Bonferroni and Tukey post hoc tests post hoc test for mean comparisons, with a significance level of p < 0.05 were used to assess statistical significance in the 3D humanized model of the periodontal gingival pocket (OriginPro 2022b, OriginLab Corporation, Northampton, MA, USA).

Techniques: Isolation, Generated, Construct, In Vivo, In Vitro